Current evidence
Research tools / Sequencing
Sequence once.
Decide what comes next.
Compare the cost of sending one sample first with sending duplicates or triplicates. Then use your read evidence to guide the next submission.
For phages expected to be 50–150 kb. A planning tool and an exploratory review screen; neither establishes a complete genome.
01
Before sequencing
Explore the cost
Expected sequencing spend / phage
$17.50
versus $30.00 when sending all three
$12.50 less per phage under these assumptions
Excludes shipping, labour and the delay between rounds. Reaching the budget does not guarantee success.
02
After the first submission
Put the yield in context
20× potential average depth
Read bases ÷ expected genome size. This assumes every read belongs to the target. Actual usable coverage can be lower and uneven; this number alone cannot tell you to stop.
03
Evidence before the next order
Review your submission combinations
Load the website-summary.json export from the sequencing analysis. Same-prefix submissions are analysed together in each selected combination, including A + B and A + B + C.
Processed in this tab only. No upload, no browser storage, and no raw sequences needed. The website displays completed analyses; it does not run an assembly.
No analysis loaded. Use the calculator above to explore a budget.
Candidate span sums all contigs, including unresolved positions; multiple contigs do not form a proven continuous genome. “Stringent support” is the fraction passing the analysis's molecular evidence checks. It is not a calibrated probability that the sequence is correct. Physical ends still require review.
| Submissions | Cost | Candidate length | Stringent support | Decision |
|---|
What did later submissions change?
These checks are retrospective. They never influence the decision made using only the currently selected submissions. The full pool shares reads with smaller combinations and is not independent ground truth.